Cell in culture need serum to survive and grow, right? Or is it? Can your cells survive without serum and why should you try?
The post Going Serum-Free: The How and Why of Removing Serum From Your Media appeared first on Bitesize Bio.
3' mRNA-seq is a NGS method to profile gene expression that is sensitive and straightforward but won’t blow your budget.
The post 3′ mRNA-Seq is Transforming Gene Expression Analysis appeared first on Bitesize Bio.
We are all familiar with bacterial cell lines as a means for protein expression and purification. But can you do the same with eukaryotic cell lines? Read on to learn some helpful tips and considerations when needing to get your hands on some eukaryotic protein.
The post How to Use a Eukaryotic System for Protein Expression & Purification appeared first on Bitesize Bio.
While DNA and RNA extraction is a pretty common technique, it isn't always the easiest. Read on to learn some top tips on how to successfully extract nucleic acids from even the toughest samples, like Arabidopsis seeds.
The post Isolating Nucleic Acids From Arabidopsis Seeds (And Other Tough Seeds) appeared first on Bitesize Bio.
Mitochondrial DNA isolation can be time-consuming and laborious. Find out how to minimise the time needed for its extraction, while ensuring fantastic results.
The post How to Isolate DNA from Mitochondria: An Energetic Guide appeared first on Bitesize Bio.